دانشگاه تحصیلات تکمیلی علوم پایه زنجان

بلوار استاد یوسف ثبوتی، پلاک 444

صندوق پستی 1159-45195 زنجان 66731-45137 ایران

دورنگار: 33155142 -024

تلفن: 33151 -024


طراحی و برنامه نويسی توسط مركز كامپيوتر دانشگاه تحصيلات تكميلي علوم پايه زنجان

خانه>دانشکده علوم زیستی>
دانشکده علوم زیستی
 
 
1- Cheraghi, N., Hosseini, M., Mohammadinejad, S., "Pore formation and the key factors in antibacterial activity of aurein 1.2 and LLAA inside lipid bilayers, a molecular dynamics study", Biochimica et Biophysica Acta (BBA) - Biomembranes, 1860: (2), 347-356, (2018).

Abstract:
Aurein 1.2 and LLAA are two antimicrobial peptides with different antibacterial activities (LLAA > Aurein 1.2), though their amino acid sequences are similar. In this manuscript, we study the key features for the different antibacterial activities of these peptides using molecular dynamics simulation. We find that in water, both peptides become disordered and LLAA is observed to have higher water-solubility, a feature which may contribute to enhancing its propensity to disrupt the bilayer and thus higher activity. Both peptides are also investigated while they are initially located inside lipid bilayer as a pre-formed vertical channel composed of five parallel copies of each peptide. LLAA demonstrates larger structural deviation from the initial helical structure and also more structural flexibility which is concluded to be a key feature in its stronger activity. In the presence of LLAA, the bilayer order is perturbed more pronouncedly and the number of water molecules penetrating into bilayer is higher. It is shown that stronger electrostatic interactions, more hydrophobic contacts and more hydrogen bonds between lipid and LLAA also lead to stronger activity of LLAA. The simulation results show instability of the barrel-stave pores for our peptides inside lipid bilayers.